中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (50): 9331-9336.doi: 10.3969/j.issn.2095-4344.2012.50.005

• 骨组织构建 bone tissue construction • 上一篇    下一篇

磁共振监测骨形态发生蛋白2及异体骨体内植入的早期血管化

邓 伟1, 2，金格勒1，殷 剑1，杨 毅1，刘晓晨3   

  • 出版日期:2012-12-09 发布日期:2012-12-09

MRI-monitored early revascularization of bone morphogenetic protein-2/allograft

  • Online:2012-12-09 Published:2012-12-09

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499

被引次数

4

摘要：

背景：课题组前期研究证实复合骨(重组人骨形态发生蛋白2及异体骨)的成骨作用不低于自体骨，并能诱导血管形成，但对早期血管形成进程及程度的监测尚缺乏有效手段。
目的：采用磁共振成像结合原位杂交方法评估复合骨在兔腰椎融合过程中诱导血管形成进程和程度。
方法：新西兰大白兔45只，随机分成3组，即复合骨组，自体骨组及单纯异体骨组。在L4、L5横突间行腰椎后路植骨融合术，分别植入非细胞型组织工程骨条，自体髂骨条及单纯异体髂骨条，于术后14，28，42 d利用磁共振成像技术获取影像学资料，选取融合标本进行原位杂交检测。
结果与结论：MRI信号强度增强率在各组间比较差有显著性意义(P < 0.05)，复合骨组信号强度增加率于42 d达到最高，术后42 d时各组间差异最大。术后14，28，42 d复合骨组血管内皮生长因子mRNA原位杂交染色表达阳性细胞数目高于自体骨及异体骨组(P < 0.05)。实验证实磁共振成像可在一定程度上监测复合骨早期血管形成，且具有无创、无辐射、高灵敏度和可定量分析的优点；复合骨早期及时建立血供促进骨愈合，从而为骨移植替代材料提供了更多选择。

Abstract:

BACKGROUND: Our previous study confirmed that the osteogenesis of the composite bone (bone morphogenetic protein-2/allograft) is not lower than that of the autogenous bone, and the composite bone can induce the angiogenesis, however, there is no effective approach to monitoring the degree of early angiogenesis process.
OBJECTIVE: To evaluate the dynamic process and degree of revascularization in rabbits undergoing posterolateral lumbar spinal fusion with composite bone via magnetic resonance perfusion imaging plus in situ hybridization method.
METHODS: Forty-five adult male New Zealand white rabbits were randomly divided into three groups: composite bone group, autogenous bone group and simple allograft group. The rabbits in each group undergoing intertransverse posterior lunbar interbody fusion at L4 and L5 respectively. The uncellular tissue engineering bone strip, self-iliac bar and simple allogeneic iliac bar were implanted into every group respectively. At 14, 28 and 42 days after surgery, the imaging data was obtained by MRI technology, then the fusion specimens were selected for the in situ hybridization detection.
RESULTS AND CONCLUSION: There were significant differences of magnetic resonance imaging signal intensity enhancement ratio between groups (P < 0.05), the ratio of the signal strength of composite bone group reached highest at 42 days after surgery, and there was significant difference between groups. At the 14, 28 and 42 days after surgery, the expression of vascular endothelial growth factor mRNA in situ hybridization staining positive cells in composite bone group was higher than that in the autogenous bone and allograft group (P < 0.05). It is helpful to reflect the dynamic process of vascularization of the composite bone via MRI with the characteristics of noninvasive, radiation-free, sensitive and quantitative; The establishment of blood supply of composite bone early and timely can promote the bone formation in the posterolateral lumbar spinal fusion, it may provide more choices for bone graft substitute materials.